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Chapters
- 00:05Title
- 01:40Transduction and Treatment of Target Cells
- 04:04Deconvolution from a Polyclonal Cell Population
- 06:16Target Identification and Validation
- 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
- 08:51Conclusion
Hier gebruiken we een menselijke LentiPlex samengevoegde bibliotheek en traditionele sequencing methoden om gen-doelstellingen het bevorderen van overleving van de cel te identificeren. Laten we zien hoe op te zetten en deconvolute een LentiPlex scherm en valideren van de resultaten.
Tags
MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes
