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Chapters
- 00:05Title
- 02:42A Two-Chamber Assay for Frog Sperm Chemotaxis
- 05:46Representative Evaluation of the Chemoattractiveness of Frog Egg Water Proteins
- 08:22Results: Evaluation of Sperm Trafficking Patterns and Behaviors in Response to a Chemotactic Stimulus
- 09:40Conclusion
Eggs and the extracellular coatings around eggs frequently release peptides, proteins and small molecules that communicate with sperm to guide them to the egg thereby promoting fertilization. Using frog sperm we describe and compare two classes of assays used to detect sperm chemoattraction – sperm accumulation assays and sperm tracking assays.
Tags
AssaysDetectingFrog SpermChemoattractionInvertebratesVertebratesQuantitative AssaysSperm MovementSperm Accumulation AssaysSwimming TrajectoriesDose Response CurvesTime CoursesNeutrophil ChemotaxisBacterial PeptidesFollicular FluidSperm Tracking AssaysLabor IntensiveSwimming PathsChemoattractant Gradient AxisCharacteristic TurnsChanges In Orientation
