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Kvantitative og Automated High-throughput Genome-wide RNAi skjermer i C. elegans</em
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JoVE Journal Biology
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Kvantitative og Automated High-throughput Genome-wide RNAi skjermer i C. elegans</em

DOI:
10.3791/3448-v

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10:58 min

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February 27, 2012

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, , ,
1Centre d’Immunologie de Marseille-Luminy,Université de la Méditerranée

Chapters

  • 00:05Title
  • 01:33Day 1, Preparation of 96-well NGM RNAi Plates, 96-DeppWell LB Plates, and RNAi Bacterial Clone Culturing
  • 03:14Day 2, Seeding RNAi Bacterial Clones onto 96-well NGM RNAi Plates
  • 04:46Days 3 and 4, RNAi Feeding of Worms and Infection of Worms with D. coniospora Spores
  • 06:02Day 5, Observation and Storage of Plates Prior to Automated Quantitative Analysis
  • 07:29Representative RNAi Results
  • 10:27Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en protokoll hjelp<em> C. elegans</em> Og RNAi fôring biblioteker som tillater automatisert måling av flere parametere som fluorescens, størrelse og tettheten av enkelte ormer i en populasjon. Vi gir ett eksempel på en skjerm for å identifisere gener involvert i anti-sopp medfødt immunitet i<em> C. elegans</em>.

Tags

QuantitativeAutomatedHigh-throughputGenome-wideRNAi ScreensC. ElegansGene FunctionKnockdownBacteriaDsRNALibrariesFunctional Genomic StudiesLaborious MethodsSemi-automated ProtocolsMicroscopic ImagingImage AnalysisRobotic HandlingCOPAS BiosortMBioLIMSData ManagementSample TrackingSolid Medium PlatesHost-pathogen InteractionsAnti-fungal Innate Immunity

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