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Chapters
- 00:05Title
- 03:11Germ Cell-transplantation Procedure
- 05:32Preparation of Donor Tissue for Xenotransplantation
- 06:57Castration of Recipient Mouse and Ectopic Xenografting
- 08:48Transplanted SSCs and Xenograft Development
- 09:54Conclusion
Protocols for germ cell transplantation and testis tissue xenografting are described. Germ cell transplantation results in donor-derived spermatogenesis in recipient testes and represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs). Testis tissue xenografting reproduces testis development and spermatogenesis of various donor species in recipient mice.
Tags
Germ Cell TransplantationMiceDr. Ralph BrinsterUniversity Of PennsylvaniaMicroinjectionSeminiferous TubulesSpermatogenesisSperm ProductionDonor-derivedBacterial β-galactosidase GeneOffspringLumen Of The Seminiferous TubulesBasement MembraneSpermatogoniaSSCsStem Cell NichePutative Spermatogonial Stem CellsTransgenic AnimalsGenetic ManipulationSertoli Cell-germ Cell InteractionSSC Homing And ColonizationSelf-renewal And Differentiation
