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Chapters
- 00:05Title
- 03:11Germ Cell-transplantation Procedure
- 05:32Preparation of Donor Tissue for Xenotransplantation
- 06:57Castration of Recipient Mouse and Ectopic Xenografting
- 08:48Transplanted SSCs and Xenograft Development
- 09:54Conclusion
Protokoller til kimcelle transplantation og testikel væv xenografting er beskrevet. Kønsceller celletransplantation resulterer i donor-afledt spermatogenesen i modtagerlandene testikler og repræsenterer et funktionelt rekonstituering analyse til identifikation af spermatogonial stamceller (SSC'er). Testis væv xenografting reproducerer testis udvikling og spermatogenese af forskellige donorarterne i recipientmus.
Tags
Germ Cell TransplantationMiceDr. Ralph BrinsterUniversity Of PennsylvaniaMicroinjectionSeminiferous TubulesSpermatogenesisSperm ProductionDonor-derivedBacterial β-galactosidase GeneOffspringLumen Of The Seminiferous TubulesBasement MembraneSpermatogoniaSSCsStem Cell NichePutative Spermatogonial Stem CellsTransgenic AnimalsGenetic ManipulationSertoli Cell-germ Cell InteractionSSC Homing And ColonizationSelf-renewal And Differentiation
