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Påvisning og isolation af cirkulerende melanom celler ved hjælp af Photoacoustic Flowmetry
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Påvisning og isolation af cirkulerende melanom celler ved hjælp af Photoacoustic Flowmetry

DOI:
10.3791/3559-v

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09:52 min

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November 25, 2011

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, , , , , ,
1Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology,University of Missouri

Chapters

  • 00:05Title
  • 01:20Blood Sample Preparation
  • 02:58Flow Chamber Construction
  • 04:32Photoacoustic Flowmetry Set-up
  • 06:42Immunocytochemistry
  • 08:39Results: Melanoma Isolation
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Vi har udviklet et flowcytometer ved hjælp af laser induceret ultralyd til at påvise cirkulerende melanom celler som en tidlig indikator for metastatisk sygdom.

Tags

DetectionIsolationCirculating Melanoma CellsPhotoacoustic FlowmetryCirculating Tumor Cells (CTCs)Metastatic DiseaseBlood SamplesCancer DiagnosisTherapy Response MonitoringPigmentPhotoacoustic WavesCustom Flow CytometerSeparationCentrifugationWhite Blood Cell LayerDensity SimilarityPhosphate Buffered Saline (PBS)FlowmeterTwo Phase FlowMicrofluidic SystemSlugs Of LiquidLaser Light Irradiation

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