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一个培养人关节软骨细胞的三维系统在滑液
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JoVE Journal Biology
A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
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一个培养人关节软骨细胞的三维系统在滑液

DOI:
10.3791/3587-v

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09:58 min

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January 31, 2012

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, ,
1Department of Anatomy and Cellular Biology,Tufts University School of Medicine, 2Department of Rheumatology,Tufts Medical Center

Chapters

  • 00:05Title
  • 01:49Preparing Human Articular Chondrocytes (HAC) for 3D Encapsulation
  • 02:51Encapsulating HACs into 3D Beads
  • 04:50Culturing Chondrocytes in Synovial Fluid
  • 06:05Harvesting HACs in Alginate Beads for Gene Expression or Histological Analysis
  • 07:363D Cultures
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

一个3D系统培养人关节软骨在滑液的高层次描述。滑液反映关节软骨的最自然的微环境，并可以很容易地获得和存储。因此，该系统可用于软骨再生研究和筛选疗法治疗关节炎。

Tags

3D SystemCulturingHuman Articular ChondrocytesSynovial FluidCartilage DestructionOsteoarthritisDisabilityCartilage RegenerationChronic PainImmobilityScaffoldsBalanced MediumIn Vivo TransplantationChondrocyte GrowthMatrix DepositionNative Joint CavitySynovial FluidAvascular Articular CartilageGrowth FactorsCytokinesEnzymesChondrocyte MetabolismHyaluronanLubricinArtificial Media

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