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酰胺氢/氘交换和MALDI - TOF质谱PAK2激活的质谱分析

JoVE Journal
Biology

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JoVE Journal Biology

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

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酰胺氢/氘交换和MALDI - TOF质谱PAK2激活的质谱分析

DOI:

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07:15 min

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November 26, 2011

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Yuan-Hao Hsu, Jolinda A. Traugh

¹Department of Chemistry,Tunghai University, ²Department of Biochemistry,University of California, Riverside

Chapters

00:05Title
02:14Identification of Pepsin-digested Pak2 Fragments
03:34Measurement of Amide H/D Exchange
05:30Time Course of Deuterium Incorporation for Inactive Pak2
06:41Conclusion

Summary

Automatic Translation

成功地利用MALDI - TOF质谱监察PAK2活化蛋白激酶的酰胺氢/氘交换。

Tags

Amide Hydrogen/deuterium Exchange MALDI-TOF Mass Spectrometry Protein-protein Interactions Protein Conformational Changes Protein Dynamics Protein-ligand Interactions Deuteration Rates Pepsin Digestion Resolution Heteronuclear Single Quantum Coherence (HSQC)NMR Aqueous Solution MALDI-TOF Detection HPLC/ESI-MS System Protein Kinase Pak2 Gamma-Pak

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