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Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
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Neuroscience
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JoVE Journal Neuroscience
Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

DOI:
10.3791/3622-v

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09:03 min

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February 07, 2012

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,
1Department of Biochemistry and Cell Biology,Rice University

Chapters

  • 00:05Title
  • 01:26Incubate Chick and Quail Eggs to the Desired Stage
  • 01:57Prepare Eggs for Windowing and Dissection
  • 06:15Grafting the Tissue
  • 07:21Immunofluorescent Detection of Quail-specific Nuclear Antigen QCPN in Donor-derived Cells in the Chimeric Embryo
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

An approach for analyzing migration and eventual fate of avian neural crest cells in quail-chick chimeric embryos is described. This method is a simple and straightforward technique for tracing neural crest cells during migration and differentiation that are otherwise difficult to distinguish within an unmanipulated chick embryo.

Tags

Neural Crest MigrationDifferentiationCross-species TransplantationAvian EmbryosQuail-chick ChimerasMigratory Neural Crest CellsEpithelial To Mesenchymal TransitionCell TypesCartilageMelanocytesNeuronsGliaMultipotent CellsRostro-caudal AxisNeighboring CellsMicroenvironmentPatterningOrganogenesis

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