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In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
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Bioengineering
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JoVE Journal Bioengineering
In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

DOI:
10.3791/3628-v

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08:56 min

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January 11, 2012

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,
1Neurosurgery,Baylor College of Medicine , 2Michael E. DeBakey Veterans Affairs Medical Center, 3Molecular & Cellular Biology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 02:23Preparation of Tissue Sections
  • 04:02Molecular Machine Assembly
  • 04:59Dual Labeling 5’OH- and 5’PO4- DNA Breaks in Tissue Sections
  • 06:41Results of Detecting DNA Breaks with a Molecular Machine
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate the assembly and application of a molecular-scale device powered by a topoisomerase protein. The construct is a bio-molecular sensor which labels two major types of DNA breaks in tissue sections by attaching two different fluorophores to their ends.

Tags

In Vitro AssemblySemi-artificial Molecular MachineDNA Damage DetectionBio-molecular MachinesArtificial Molecular MachinesMolecular MotorsNanoworld EnvironmentsNanotechnology ProductsBio-enabled ConstructDesigner Fluorescent Enzyme-substrate ComplexMolecular MachineFluorescent Dual HairpinLabeling DNA Da

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