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Chapters
- 00:00Title
- 00:47Introduction
- 02:08Materials
- 02:36Excision of the Corneoscleral Button
- 03:29Introduction of Cornea into Organotypic Culture
- 04:22Infection with HSV-1 and Treatment with Experimental Drugs
- 05:25Medium for Plaque Assays
- 05:45DNA, RNA, Protein, or Cells from Corneal Epithelium
- 06:48Tissue Samples for Immunohistochemistry or Immunofluorescence
- 07:23Conclusion
Tags
Ex Vivo Organotypic Corneal ModelAcute Epithelial Herpes Simplex Virus Type I InfectionHerpes KeratitisHSV-1Cornea-derived BlindnessInfection-associated BlindnessCorneal EpitheliumCorneal StromaCorneal EndotheliumScarringThinningOpacityIn Vitro ModelIn Vivo ModelCultured MonolayersPetri DishPhysiological SystemEx Vivo ModelOrganotypically Maintained Culture
