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Vurdering af GFP Expression og levedygtighed Brug af Tali Billed-Based cytometer

JoVE Journal
Biology

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JoVE Journal Biology

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer

Vurdering af GFP Expression og levedygtighed Brug af Tali Billed-Based cytometer

DOI:

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10:58 min

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November 17, 2011

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Krissy Remple, Laurel Stone

¹Department of Molecular and Cell Biology,Life Technologies , ²Life Technologies

Chapters

00:05Title
01:26Staining Cells with Tali Viability Kit – Dead Cell Red
02:16Cell Viability Assay Using the Tali Image-Based Cytometer
05:32Setting Thresholds and Gates
09:33Tali Image-Based Cytometry vs. Flow Cytometry
10:12Conclusion

Summary

Automatic Translation

Denne protokol beskriver, hvordan du udfører cellernes levedygtighed og fluorescens udtryk analyser ved hjælp af Tali Billed-Based cytometer.

Tags

GFP Expression Viability Assessment Tali Image-based Cytometer Flow Cytometry Fluorescence Microscopy Quantitative Measurements Visualization Image-based Cytometry Cell Viability Assay GFP Expression Assay Tali Instrument Benchtop Assay Platform Suspension Cell-based Assays Apoptosis Analysis Propidium Iodide

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