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Immunohistological Merking av mikrotubuli i Sensory Neuron dendritter, trakéene, og musklene i Drosophila Larva Body Wall
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Chapters
- 00:05Title
- 01:11Larval Dissection
- 02:56Fixation of Larval Fillets
- 03:59Blocking, Staining, and Mounting of Larval Fillets
- 05:56Fluorescent Staining
- 07:22Conclusion
For å forstå hvordan komplekse celle former, som nevrale dendritter, er oppnådd under utvikling, er det viktig å kunne nøyaktig analysen microtubuli organisasjon. Her beskriver vi en robust immunohistological merking metode for å undersøke microtubuli organisering av dendrittiske arborization nervecellen sensoriske dendritter, luftrøret, muskel, og andre<em> Drosophila</em> Larva kroppen veggen vev.
Tags
Immunohistological LabelingMicrotubulesSensory Neuron DendritesTracheaeMusclesDrosophila Larva Body WallCell ShapesMicrotubule OrganizationTube MorphogenesisDendritic ArborizationNeuron-class-specific MechanismsDendritic DifferentiationDegenerationCytoskeletal OrganizationImmunological Labeling MethodIn Vivo Microtubule OrganizationFirst Instar LarvaSensory Neuron Dendrite OutgrowthBranching OrganizationMuscles LabelingTrachea Labeling
