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Hybridisering<em> In situ</em> Af spytkirtler, æggestokke og embryoner af Vector Myg
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Chapters
- 00:05Title
- 01:25In situ Hybridization
- 03:27Glycerol Mounting
- 05:30Dechorionation, Fixation, and Endochorion Disruption
- 08:04Peeling
- 09:44Representative In situ Results
- 10:36Conclusion
Tidsmæssige og rumlige genekspressionsanalyser har en afgørende rolle i funktionel genomforskning. Whole-mount hybridisering<em> In situ</em> Er nyttig til bestemmelse af lokaliseringen af transkripter i væv og subcellulære rum. Her har vi skitsere en hybridisering<em> In situ</em> Protokol med modifikationer for specifikke målvæv i myg.
Tags
Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization
