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Isolering av basal celler og submukøst Gland kanalsystem celler fra mus Trachea
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JoVE Journal Biology
Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

Isolering av basal celler og submukøst Gland kanalsystem celler fra mus Trachea

DOI:
10.3791/3731-v

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11:52 min

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September 14, 2012

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, , , ,
1Department of Pediatrics,David Geffen School of Medicine at UCLA

Chapters

  • 00:05Title
  • 01:45Dissection of Trachea
  • 02:46Cleaning and Cutting the Trachea
  • 03:45Enzyme Digestion and Processing into Single Cell Suspension
  • 06:42Staining for FACS and Sorting
  • 07:23Processing Sorted Cells for in vitro Culture and in vivo Model
  • 09:40Representative Data
  • 11:19Conclusion

Summary

Automatic Translation

Automatic Translation

Her viser vi vår protokoll for isolering av basal og submucosal kjertel duct celler fra mus tracheas. Vi også demonstrere metoden av å injisere stamceller i dorsal musen fett puten å skape en<em> In vivo</em> Modell av submucosal kjertel gjenfødelse.

Tags

IsolationBasal CellsSubmucosal Gland Duct CellsMouse TracheaLarge AirwaysRepair MechanismStem CellsIn Vitro Model SystemsIn Vivo Model SystemsMechanisms Of Repair And RegenerationAirway DiseasesCystic FibrosisAsthmaChronic Obstructive Pulmonary Disease

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