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Journal
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Immunology and Infection
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从初级人类的幼稚和记忆T细胞的诱导调节性T细胞的生成
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Please note that all translations are automatically generated.
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从初级人类的幼稚和记忆T细胞的诱导调节性T细胞的生成
DOI:
10.3791/3738-v
•
14:23 min
•
April 16, 2012
•
Gavin I. Ellis
,
Mary Catherine Reneer
,
Alejandra Catalina Vélez-Ortega
,
Andrea McCool
,
Francesc Martí
1
Department of Microbiology, Immunology and Molecular Genetics
,
University of Kentucky
Chapters
00:05
Title
02:22
Isolation of Human Peripheral Blood Mononuclear Cells (PBMCs) from Buffy Coat
03:58
Magnetic Negative Selection of T Cells from PBMCs using EasySep Enrichment Kit
06:16
Cell Culture Conditions to Induce Regulatory T Cells
07:11
Fluorescence-activated Cell Sorting of Three Populations of T Cells
08:28
Suppression Assay
10:32
Representative Sorting and Suppression Assay Results
13:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了从一个单一的人献血的监管,内存和幼稚T细胞产生的一种方法。极化调节性T细胞可以比较多种遗传同质性,这里还详细介绍了包括抑制法与遗传和功能应用中的其他子集。
Tags
Induced Regulatory T Cells
Primary Human Naive T Cells
Memory T Cells
Immunosuppressive CD4+ Regulatory T Cells
Peripheral Tolerance
Immunologic Homeostasis
Self Antigens
Commensal Antigens
Treg Biology
Therapeutic Injection Of Tregs
Murine Models
Colitis
Type I Diabetes
Rheumatoid Arthritis
Graft Versus Host Disease
Ex Vivo Expansion
Peripheral Blood Mononuclear Cells
CD4+ T Cells
In Vitro Cell Culture
Fluorescence Activated Cell Sorting (FACS)
Forkhead Box P3 (FoxP3)
Activation-induced Transcription Factor
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在
从幼稚的CD4
+
T细胞
的
人CD4
+
FOXP3
+
诱导调节性T细胞(iTregs的)使用含有TGF-β-协议的
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分化
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