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Chapters
- 00:05Title
- 01:32Preparation of 3H-cholesterol
- 01:53Label Cellular Cholesterol
- 02:37Equilibrium Incubation
- 03:19Cholesterol Efflux Incubation
- 04:10Processing Samples
- 05:45Data Analysis
- 06:11Representative Results: Assaying Modulators of Cholesterol Efflux in Macrophages
- 07:32Conclusion
Den cholesterol-assay er designet til at kvantificere graden af cholesterol efflux fra dyrkede celler og kapaciteten af plasma acceptorer til at modtage cholesterol frigivet fra cellerne. Analysen består af mærkning af celler med kolesterol, ligevægt af kolesterol blandt intracellulære pools og frigivelse af kolesterol til et ekstracellulært acceptor.
Tags
Cholesterol Efflux AssayCholesterol ContentCellular MembranesApoptosisNecrosisIntracellular SynthesisPlasma LipoproteinsCholesterol SynthesisCholesterol UptakeDeficiencies Of CholesterolExcessive CholesterolHepatocytesAdrenocortical CellsCholesteryl EstersATP Binding Cassette Transporter Proteins A1 (ABCA1)ATP Binding Cassette Transporter Proteins G1 (ABCG1)Scavenger Receptor Type B1High Density Lipoprotein (HDL)Apolipoprotein A-I
