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Multiphoton Mikroskopi af Cleared Mouse Brain udtrykker YFP
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Neuroscience
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JoVE Journal Neuroscience
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Multiphoton Mikroskopi af Cleared Mouse Brain udtrykker YFP

DOI:
10.3791/3848-v

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10:03 min

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September 23, 2012

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, , ,
1Department of Biomedical Engineering,Yale University, 2Department of Biomedical Engineering,Louisiana Tech University

Chapters

  • 00:00Title
  • 03:08Animal Perfusion and Whole Mouse Brain Clearing
  • 06:57Microscope Setup
  • 08:12Representative Results and Conclusion

Summary

Automatic Translation

Automatic Translation

Multiphoton microscopy af hele muse organer er mulig ved optisk fjerne organ før billeddannelse, men ikke alle protokoller bevare det fluorescerende signal af fluorescerende proteiner. Brug af et optisk clearing metode med ethanol-baseret dehydrering og benzylalkohol: benzylbenzoat clearing, viser vi i høj opløsning multiphoton billeder af hele musehjerne udtrykker YFP.

Tags

Multiphoton MicroscopyCleared Mouse BrainYFP ExpressionIntrinsic FluorescenceSecond Harmonic Generation (SHG)Optically ClearingMethanol DehydrationBenzyl Alcohol:benzyl Benzoate (BABB)Fluorescent SignalOptical Clearing ProtocolsEthanol Graded SeriesFluorescent ProteinsMultiphoton ImagingHigh-resolution ImagesNeurons

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