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Biology
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Metode for isolering og identifisering av mRNA, microRNAs og protein komponenter av ribonucleoprotein Komplekser fra Cell ekstrakter med RIP-Chip
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
Metode for isolering og identifisering av mRNA, microRNAs og protein komponenter av ribonucleoprotein Komplekser fra Cell ekstrakter med RIP-Chip
DOI:
10.3791/3851-v
•
13:34 min
•
September 29, 2012
•
Garrett M. Dahm
2
,
Matthew M. Gubin
2
,
Joseph D. Magee
,
Patsharaporn Techasintana
2
,
Robert Calaluce
,
Ulus Atasoy
2,3
1
Molecular Microbiology and Immunology
,
University of Missouri
,
2
Department of Surgery
,
University of Missouri
,
3
Child Health
,
University of Missouri
Chapters
00:00
Title
01:43
Harvesting RNP Complex
03:59
Preparation of Isolation Materials
06:40
Immunoprecipitation
08:42
DNase and Proteinase K Treatment and RNA Precipitation
12:11
Representative Results
12:49
Conclusions
Summary
Automatic Translation
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Automatic Translation
En trinnvis protokollen til å isolere og identifisere RNA knyttet komplekser gjennom RIP-Chip.
Tags
Isolation
Identification
MRNAs
MicroRNAs
Protein Components
Ribonucleoprotein Complexes
RIP-Chip
High-throughput Sequencing
Microarray Analysis
Global Gene Expression Analysis
Post-transcriptional Gene Regulation
RNA Binding Proteins (RBP)
MRNA Localization
Stability
Translation
Ribonucleoprotein (RNP) Complex
De Novo MRNA Targets
Cellular Extracts
Protein Output
RNP Immunoprecipitation-microarray (RIP-Chip)
Experimental Conditions
Post-transcriptional Gene Regulation Mechanisms
Ribonucleoprotein Interactions
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