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Uttrykk og rensing av Cystisk fibrose transmembrane Conduktans Regulator Protein i Saccharomyces cerevisiae
JoVE Journal
Biology
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JoVE Journal Biology
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

Uttrykk og rensing av Cystisk fibrose transmembrane Conduktans Regulator Protein i Saccharomyces cerevisiae

DOI:
10.3791/3860-v

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14:56 min

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March 10, 2012

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, , ,
1Faculty of Life Sciences,University of Manchester

Chapters

  • 00:05Title
  • 03:42Screening Transformants
  • 07:35Large-scale Fermenter Culture
  • 11:37Representative Yeast Transformation Results
  • 13:27Conclusion

Summary

Automatic Translation

Automatic Translation

Forsøk på å uttrykke den cystisk fibrose transmembrane konduktans regulator (CFTR) i<em> Saccharomyces cerevisiae</em> Har, til nå, gitt relativt lave mengder protein. Denne protokollen og de tilhørende reagenser distribueres via Cystisk fibrose Foundation bør tillate utarbeidelse av milligram mengder av dette "vanskelige" eukaryot membran protein.

Tags

Cystic Fibrosis Transmembrane Conductance RegulatorCFTR ProteinExpressionPurificationSaccharomyces CerevisiaeChloride ChannelCystic FibrosisProtein StructureProtein ActivityRecombinant ProteinYeast S. CerevisiaeProteolysis ProblemsCell Growth ConditionsInduction ConditionsReagentsUS Cystic Fibrosis Foundation

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