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Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
DOI:

08:08 min

April 05, 2012

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Chapters

  • 00:05Title
  • 01:30Making Electrodes
  • 02:41Isolation of Olfactory Receptor Neurons
  • 04:18Recordings
  • 06:28Results: Odorant-induced Responses Recorded from ORNs using the Suction Pipette Technique
  • 07:46Conclusion

Summary

Automatic Translation

Olfactory receptor neurons (ORNs) convert odor signals first into a receptor current that in turn triggers action potentials that are conveyed to second order neurons in the olfactory bulb. Here we describe the suction pipette technique to record simultaneously the odorant-induced receptor current and action potentials from mouse ORNs.

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