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Chapters
- 00:05Title
- 01:55Preparing the STLV Bioreactor
- 04:01Preparing Microcarrier Beads
- 04:35Seeding Epithelial Cells and Beads into the STLV Bioreactor
- 06:45Culturing, Sampling, and Photo Documentation of Cultures
- 08:19Transferring and Harvesting Aggregates
- 09:273D-aggregates and Biology of Human Vaginal Epithelial Cells
- 11:59Conclusion
Et roterende cellekultur, der tillader epitelceller til at vokse under fysiologiske betingelser, hvilket resulterer i 3-D cellulær aggregatdannelse er beskrevet. Aggregaterne dannes display<em> In vivo</em>-Lignende egenskaber, ikke er observeret i traditionelle kultur modeller og tjene som et mere præcist organotypiske modelsystem for en lang række videnskabelige undersøgelser.
Tags
Rotating Wall Vessel Bioreactor3D Epithelial Cell ModelsIn Vitro Cell CultureGrowth EnvironmentConventional Cell Culture SystemsPhysiologically Relevant MicroenvironmentRotating Wall Vessel (RWV) Bioreactor TechnologyOrganotypic ModelsExternal StimuliSuspension Culture SystemLow Physiological Fluid Shear ConditionsHigh-aspect Rotating Vessel (HARV)Slow-turning Lateral Vessel (STLV)Aeration Source
