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Chapters
- 00:05Title
- 01:55Preparing the STLV Bioreactor
- 04:01Preparing Microcarrier Beads
- 04:35Seeding Epithelial Cells and Beads into the STLV Bioreactor
- 06:45Culturing, Sampling, and Photo Documentation of Cultures
- 08:19Transferring and Harvesting Aggregates
- 09:273D-aggregates and Biology of Human Vaginal Epithelial Cells
- 11:59Conclusion
Un sistema de cultivo celular giratorio que permite a las células epiteliales de crecer bajo condiciones fisiológicas resultantes en 3-D formación de agregados celulares se describe. Los agregados generado pantalla<em> En vivo</em> Características similares no se observó en los modelos de cultivo convencionales y servir como un sistema modelo más exacto organotípico para una multitud de investigaciones científicas.
Tags
Rotating Wall Vessel Bioreactor3D Epithelial Cell ModelsIn Vitro Cell CultureGrowth EnvironmentConventional Cell Culture SystemsPhysiologically Relevant MicroenvironmentRotating Wall Vessel (RWV) Bioreactor TechnologyOrganotypic ModelsExternal StimuliSuspension Culture SystemLow Physiological Fluid Shear ConditionsHigh-aspect Rotating Vessel (HARV)Slow-turning Lateral Vessel (STLV)Aeration Source
