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Chapters
- 00:05Title
- 01:44Pretreatment of Cells and Metaphase Preparation
- 05:16Pepsin Pretreatment of Slides
- 07:32Chromosome and Probe Denaturation and Hybridization
- 09:00Fluorescent Probe Design
- 10:53Representative Results from Spectral Karyotyping of Mouse and Human Chromosomes
- 12:31Conclusion
Cariotipo spettrale (SKY) è una tecnica avanzata citogenetica per individuare aberrazioni genomiche e cromosomiche. Questa tecnica si avvale di sonde cromosomiche pittura, che permettono la classificazione di tutti i cromosomi. SKY può anche identificare aberrazioni cromosomiche complesse e difetti segregazione nei topi e nell'uomo con varie patologie, tra cui la malattia del rene policistico.
Tags
Spectral KaryotypingChromosome AbnormalitiesHumansMicePolycystic Kidney DiseaseBanding PatternClassical Banding TechniqueReliable IdentificationComplex Chromosomal AberrationsCancerSpectral Karyotyping (SKY)Multicolor Fluorescence In-situ Hybridization (FISH)Spectral MicroscopeCytogenetic AnalysisGenetic DiseasesMalignanciesMulticolor Fluorescently-labelled DNA ProbesDegenerate Oligonucleotide PrimersPCRUnique Spectral ColorIn-situ HybridizationFluorescent DyesRhodamineTexas RedCy5FITCCy5.5Chromosome Specific Probes
