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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
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Chapters
- 00:05Title
- 01:39Physics of ITP
- 03:20Device Cleaning and Preparation
- 04:43Fluorophore Focusing in Peak Mode ITP
- 05:30Extraction and Purification of Nucleic Acids from Cultured E. Coli
- 07:21Separation of Amino Acides with Cationic Plateau Mode ITP and Non-Focusing Tracer (NFT) for Visualization
- 08:33Representative ITP Results
- 09:54Conclusion
Isotachophoresis (ITP) is a robust electrokinetic separation and preconcentration technique with applications ranging from toxin detection to sample preparation. We review the physical principles of ITP and the methodology of applying this technique to two specific example applications: separation and detection of small molecules and purification of nucleic acids from cell culture lysate.
Tags
On-chip IsotachophoresisSeparation Of IonsPurification Of Nucleic AcidsElectrokinetic TechniquesMicroscale ApplicationsFluidic ProcessesElectrophoretic ProcessesIsotachophoresis (ITP)PreconcentrationIonic MobilityUnlabeled Ionic MoleculesSample PreparationExtraction And Purification Of Nucleic AcidsComplex MatricesCell CultureUrineBloodElectric FieldBuffersAnionic Analytes
