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En væskefase Affinity Capture Assay hjelp av magnetiske perler å studere protein-Protein Interaction: Den poliovirus-Nanobody Eksempel
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JoVE Journal Biology
A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

En væskefase Affinity Capture Assay hjelp av magnetiske perler å studere protein-Protein Interaction: Den poliovirus-Nanobody Eksempel

DOI:
10.3791/3937-v

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08:10 min

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May 29, 2012

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, ,
1Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research,Vrije Universiteit Brussel

Chapters

  • 00:05Title
  • 02:27Preparation of the Magnetic Beads
  • 03:00Affinity Capture Assay
  • 04:18Recycling the Beads
  • 05:44Interpretation of the Results
  • 06:13Results: Liquid Phase Affinity Capture Assay using PVSS38C Nanobody
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

I denne artikkelen, er en enkel, kvantitativ, væskefase affinitet fangst analysen presentert. Det er en pålitelig teknikk basert på samspillet mellom magnetiske kuler og merkede proteiner (f.eks nanobodies) på den ene siden og affinitet mellom tagget protein og en andre, merket protein (f.eks poliovirus) på den andre.

Tags

Liquid Phase Affinity Capture AssayMagnetic BeadsProtein-protein InteractionTagged ProteinLabeled ProteinCobalt Coated Magnetic BeadsRecognition Of The TagBound FractionUnbound FractionConformational Conversion Sensitive ProteinsPoliovirus-nanobody InteractionELISASolid Surface Attachment

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