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Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines

Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines

DOI:
10.3791/3960-v

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12:21 min

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August 01, 2012

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, , , ,
1U.S. Army Medical Research Institute of Infectious Diseases

Chapters

  • 00:05Title
  • 02:28NALT Collection and Culturing
  • 04:25Surgical Ablation of NALT
  • 07:05Preparing NALT for Histology and Assessing the Success of NALT Surgery
  • 09:42Histological Analysis and Antigen Production after Vaccine Administration
  • 11:26Conclusion

Summary

Automatic Translation

Automatic Translation

Methods to examine contributions of the nasopharyngeal-associated lymphoreticular tissues (NALT) to nasal and systemic immune responses of mice to intranasal vaccines are described. We demonstrate a surgical procedure to establish a NALT-dependent mouse model and ex vivo cultures of extracted NALT.

Tags

NALTImmunityNasal SinusesMucosal-associated Lymphoid SystemB LymphocytesT LymphocytesAntigen-presenting Dendritic CellsEpithelial CellsM-cellsRespiratory PathogensWaldeyer’s RingAnimal ModelImmune ResponsesNasal SinusesSingle-cell SuspensionsMononuclear CellsEx Vivo Culturing

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