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Isolering og dyrkning af Rotte Embryonale Neurale Celler: En hurtig protokol
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

Isolering og dyrkning af Rotte Embryonale Neurale Celler: En hurtig protokol

DOI:
10.3791/3965-v

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10:51 min

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May 24, 2012

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,
1LSU Health Sciences Center – New Orleans, 2Medical School and Stanley S. Scott Cancer Center

Chapters

  • 00:05Title
  • 01:42Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
  • 03:03PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
  • 04:07Neuronal Dissection and Culture
  • 09:40Results: Isolation and Culture of Rat Embryonic Neural Cells
  • 10:27Conclusion

Summary

Automatic Translation

Automatic Translation

Beskriver vi en hurtig metode til isolering og dyrkning hippocampale og corticale neuroner fra gnavere embryoer. Denne protokol tillader os at udføre eksperimenter, hvor næsten rene neuronale kulturer er påkrævet.

Tags

IsolationCultureRat Embryonic Neural CellsQuick ProtocolDissociationHippocampal NeuronsCortical NeuronsE15-17 Rat EmbryosMouse Primary NeuronsHuman Primary NeuronsNeural ProgenitorsSerum-free MediumNucleofectionImmunocytochemistryNucleic Acids PreparationElectrophysiologyLentiviral TransductionCalcium Phosphate TransfectionLipid-based Transfection

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