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Chapters
- 00:05Title
- 02:04Anesthesia and Monitoring
- 08:00Preparation of the Cremaster Muscle
- 11:27Intravital Setup
- 13:15Offline Video Playback Analysis
- 13:59Conclusion
Digital intravital epifluorescens-mikroskopi af postkapillære venuler i den cremasteric mikrocirkulation er en bekvem metode til at få indblik i leukocyt-endotel-interaktion<em> In vivo</em> I iskæmi-reperfusionsskade (IRI) af tværstribet muskelvæv. Vi her give en detaljeret protokol til sikkert at udføre teknikken og diskutere dens anvendelsesmuligheder og begrænsninger.
Tags
Real-timeDigital ImagingLeukocyte-endothelial InteractionIschemia-reperfusion InjuryIRIRat Cremaster MusclePathological ConditionsCerebral StrokeMyocardial InfarctionIntestinal IschemiaTransplantCardiovascular SurgeryReactive Oxygen SpeciesComplement SystemMicrovascular PermeabilityLeukocyte ActivationAdhesion MoleculesChemoattractantsSelectinsCounter LigandsIntercellular Adhesion MoleculesVascular Cellular Adhesion MoleculesIntravital Microscopy
