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Chapters
- 00:05Title
- 01:20Assembling Reagents and Materials
- 02:24A Polymerase Chain Reaction: Set-up
- 04:24Basic PCR Protocol
- 05:12Programming the Thermal Cycler
- 06:29Troubleshooting
- 07:27Amplification of Gal3
- 08:38Conclusion
PCR has emerged as a common technique in many molecular biology laboratories. Provided here is a quick guide to several conventional PCR protocols. Because each reaction is a unique experiment, optimal conditions required to generate a product vary. Understanding the variables in a reaction will greatly enhance troubleshooting efficiency, thereby increasing the chance to obtain the desired result.
Tags
Polymerase Chain ReactionPCRBasic ProtocolTroubleshootingOptimization StrategiesBiological SciencesTechnological AdvancesMicrobiologyAnton Van LeeuwenhoekMicroscopeProkaryotesKeppe And CoworkersKary MullisCetus CorporationThermal Stable DNA PolymeraseTaq DNA PolymeraseAmplification TechniqueAmpliconDNA TemplateTarget SequenceSpurious ResultsAgarose Gels
