A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Medicine,Weill Cornell Medical College , 2Department of Oral Biology,University of Missouri-Kansas City-School of Dentistry, 3Department of Pharmacology and Toxicology,University of Missouri Kansas City- School of Pharmacy, 4Regional Hospital,Bamenda, NWP, Cameroon, 5Mezam Polyclinic HIV/AIDS Treatment Center, Cameroon, 6Institute for Human Genetics and Biochemistry
Chapters
- 00:05Title
- 02:31Preparation of Working Solutions
- 03:51Saliva Collection and Handling
- 04:57Microplate Set-up
- 06:55Calculating the alpha1PI Index
- 07:30Representative Assay Results
- 10:11Conclusion
Un metodo enumerazione CD4, la α-test, viene descritto che utilizza saliva intera per fornire rapidi e precisi conta di CD4. Le α-test centesimi i costi ed elimina la necessità di una formazione tecnica, reagenti costosi come gli anticorpi monoclonali, strumentazione, refrigerazione, trasporto dei campioni, nonché di raccolta e trattamento del sangue.
Tags
Affordable CD4 EnumerationHIV Disease MonitoringResource-limited RegionsTime And Temperature RestrictionsTechnical SophisticationCost Of ReagentsMonoclonal AntibodiesCell-free CorrelatesCD4 Counts CalculationPhysiological RelevanceStem CellsCXCL12CXCR4Cell Surface Human Leukocyte Elastase (HLECS)α1proteinase Inhibitor (α1PI)HIV-1 Disease Processes
