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Chapters
- 00:05Title
- 02:39Manual Quantitative Fitness Analysis Protocol: Culturing Yeast Strains
- 03:11Fully Automated Quantitative Fitness Analysis Protocol
- 04:48Image and Experimental Metadata Capture: Automated Quantitative Fitness and Data Analysis
- 09:32Representative Fitness Analysis Results
- 10:40Conclusion
Kvantitative Fitness Analyse (QFA) er en supplerende serie af eksperimentelle og beregningsmæssige metoder til estimering af mikrobielle kultur fitnesses. QFA estimerer effekten af genetiske mutationer, lægemidler eller andre anvendte behandlinger for mikrobe vækst. Forsøgene skalering af fokuserede analyse af enkelte kulturer til tusindvis af parallelle kulturer kan udformes.
Tags
Quantitative Fitness AnalysisQFAFitness ComparisonMicrobial CulturesGenetic InteractionDrug ScreensGrowth CurvesQuantitative Cell Density EstimatesFitness MeasuresGenetic Interaction StrengthsDrug SensitivitiesSubstrate AgarSmall MoleculesAntibioticsNutrientsUV IrradiationTemperatureGrowth Rate EstimatesSpectrophotometric MeasurementHigh Throughput
