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Chapters
- 00:05Title
- 00:58Adenoviral Transduction and Culturing of Rat Islets
- 03:26Insulin Secretion Assay
- 06:13Thymidine Incorporation Assay
- 07:49Beta Cells Overexpressing “Gene #6”
- 09:09Conclusion
Denne protokol tillader en at identificere faktorer, der modulerer funktionelle beta-cellemasse til at finde potentielle terapeutiske mål for behandling af diabetes. Den protokol består af en strømlinet metode til at vurdere islet replikation og beta celle funktion i isolerede rotte-øvævsceller efter manipulering af genekspression med adenovirus.
Tags
ReplicationBeta Cell FunctionAdenovirally-transduced Isolated Rodent IsletsGlucose HomeostasisInsulinGlucagonPancreatic Beta CellsAlpha CellsFunctional Beta Cell MassNutrient LoadDiabetesAutoimmune AttackType 1 DiabetesType 2 DiabetesInsulin SecretionMolecular PathwaysTherapeutic TargetsBeta Cell GrowthImpairing Beta Cell FunctionOncogenesTarget Genes
