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Vurdere Replication og betacellefunksjon i Adenovirally-transduced Isolert gnager Holmer
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JoVE Journal Medicine
Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets

Vurdere Replication og betacellefunksjon i Adenovirally-transduced Isolert gnager Holmer

DOI:
10.3791/4080-v

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09:31 min

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June 25, 2012

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, , ,
1Department of Pediatrics,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine

Chapters

  • 00:05Title
  • 00:58Adenoviral Transduction and Culturing of Rat Islets
  • 03:26Insulin Secretion Assay
  • 06:13Thymidine Incorporation Assay
  • 07:49Beta Cells Overexpressing “Gene #6”
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen gjør det mulig å identifisere faktorer som modulerer funksjonell beta cellemasse å finne potensielle terapeutiske mål for behandling av diabetes. Protokollen består av en strømlinjeformet metode for å vurdere holmen replikering og betacellefunksjon i isolerte rotte holmer etter manipulering av genuttrykk med adenoviruses.

Tags

ReplicationBeta Cell FunctionAdenovirally-transduced Isolated Rodent IsletsGlucose HomeostasisInsulinGlucagonPancreatic Beta CellsAlpha CellsFunctional Beta Cell MassNutrient LoadDiabetesAutoimmune AttackType 1 DiabetesType 2 DiabetesInsulin SecretionMolecular PathwaysTherapeutic TargetsBeta Cell GrowthImpairing Beta Cell FunctionOncogenesTarget Genes

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