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Chapters
- 00:05Title
- 01:36Expression and Purification of Recombinant Tagged Fusion Proteins in Bacteria
- 05:21Cell Culture and Cell Lysate Preparation
- 06:03In vitro Assembly of a CFTR-containing Macromolecular Complex (CFTR-PDZK1-MRP4)
- 08:23Representative Results
- 09:06Conclusion
Cystisk fibrose transmembran konduktans regulator (CFTR), en epitelcelle chlorid-kanal, er blevet rapporteret at interagere med forskellige proteiner og regulere vigtige cellulære processer, deriblandt CFTR PDZ motiv-medierede interaktioner er blevet godt dokumenteret. Denne protokol beskriver de metoder vi har udviklet til at samle en PDZ-afhængig CFTR makromolekylære signalering kompleks<em> In vitro</em>.
Tags
In Vitro AnalysisPDZ-dependent CFTR Macromolecular Signaling ComplexesCFTRChloride ChannelTransepithelial Fluid HomeostasisCystic FibrosisSecretory DiarrheaCFTR Cl- ChannelPhysical InteractionsFunctional InteractionsTransportersIon ChannelsReceptorsKinasesPhosphatasesSignaling MoleculesCytoskeletal ElementsCFTR Carboxyl Terminal TailPSD95/Dlg1/ZO-1 (PDZ) MotifScaffold Proteins
