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Chapters
- 00:05Title
- 01:36Expression and Purification of Recombinant Tagged Fusion Proteins in Bacteria
- 05:21Cell Culture and Cell Lysate Preparation
- 06:03In vitro Assembly of a CFTR-containing Macromolecular Complex (CFTR-PDZK1-MRP4)
- 08:23Representative Results
- 09:06Conclusion
Cystisk fibros transmembran konduktans-regulator (CFTR), en epitelial kloridkanal, har rapporterats interagera med olika proteiner och reglera viktiga cellulära processer, däribland de CFTR PDZ-motivet-medierade interaktioner har dokumenterats väl. Detta protokoll beskriver metoder vi utvecklat för att montera en PDZ-beroende CFTR makromolekylära signalering komplex<em> In vitro</em>.
Tags
In Vitro AnalysisPDZ-dependent CFTR Macromolecular Signaling ComplexesCFTRChloride ChannelTransepithelial Fluid HomeostasisCystic FibrosisSecretory DiarrheaCFTR Cl- ChannelPhysical InteractionsFunctional InteractionsTransportersIon ChannelsReceptorsKinasesPhosphatasesSignaling MoleculesCytoskeletal ElementsCFTR Carboxyl Terminal TailPSD95/Dlg1/ZO-1 (PDZ) MotifScaffold Proteins
