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Chapters
- 00:05Title
- 01:21Production of Oregon Green 488-gelatin Coated Coverslips
- 04:48Plating Cells on Oregon green 488-gelatin Coated Coverslips
- 05:33Quantification of Fluorescent Gelatin Degradation by Measuring Normalized Matrix Degradation
- 09:28Results: Invadopodia-mediated ECM Proteolysis
- 13:23Conclusion
Vi beskriver den prototypiske fremgangsmåte for fremstilling mikroskop dekkglass belagt med fluorescerende gelatin for å visualisere invadopodia-mediert matrise nedbrytning. Beregningsorientert teknikker ved hjelp av tilgjengelig programvare presenteres for å tallfeste resulterende nivåer av matrix proteolyse av enkeltceller i en blandet befolkning og for flercellede grupper som omfatter hele mikroskopiske felt.
Tags
Quantitative MeasurementInvadopodia-mediatedExtracellular Matrix ProteolysisSingle And Multicellular ContextsCellular InvasionDevelopmentHomeostasisMalregulated InvasionCell MovementPathological ProcessesInflammationCardiovascular DiseaseTumor Cell MetastasisProteolytic DegradationExtracellular Matrix (ECM)Epithelial Basement MembraneEndothelial Basement MembraneVentral Actin-rich Membrane Protrusive StructuresInvadopodiaMatrix Metalloproteinases (MMPs)Tumor CellsIn Vitro AnalysisECM DegradationFluorescent MicroscopyDye-labeled Matrix ProteinsGlass CoverslipsEvaluating Matrix ProteolysisCellular Invasive Potential
