Journal
/
Medicine
/
The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson’s Disease
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson’s Disease

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson’s Disease

DOI:
10.3791/4228-v

•

15:09 min

•

October 03, 2012

•

,
1German Center for Neurodegenerative Diseases,DZNE, 2Laboratory of Functional Neurogenomics, Department of Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research,University of Tübingen

Chapters

  • 00:05Title
  • 01:47Skin Biopsy and Culturing of Human Fibroblasts
  • 03:29Preparation of Human Fibroblasts for Live Cell Imaging Microscopy
  • 04:59Measurement of Mitochondrial Function
  • 06:36Image Analysis of MMP-dyed Fibroblasts
  • 08:18Measurement of Mitochondrial Morphology
  • 09:25Image Analysis of Mitochondrial Morphology in Fibroblasts
  • 11:15Measurement of Mitochondrio-lysosomal Colocalization
  • 13:01Live Cell Images of Human Fibroblasts
  • 14:43Conclusion

Summary

Automatic Translation

Automatic Translation

Fibroblasts from patients carrying mutations in Parkinson's disease-causing genes represent an easily accessible ex vivo model to study disease-associated phenotypes. Live cell imaging gives the opportunity to study morphological and functional parameters in living cells. Here we describe the preparation of human fibroblasts and subsequent monitoring of mitochondrial phenotypes.

Tags

Primary Human FibroblastsMonitoringMitochondrial PhenotypesParkinson’s DiseaseAgeingRisk FactorNeurodegenerationIn Vitro ModelsMolecular PathwaysEx Vivo ModelsDisease-associated MutationsSkin BiopsiesCulturing Human FibroblastsMicroscopic TechniquesMitochondrial FunctionMitochondrial Dynamics

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4228?article_type=v

Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
check_url/4228?article_type=v

Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson’s Disease
check_url/4228?article_type=v

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson’s Disease
check_url/4228?article_type=v

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
check_url/4228?article_type=v

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
check_url/4228?article_type=v

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease
check_url/4228?article_type=v

Gene-environment Interaction Models to Unmask Susceptibility Mechanisms in Parkinson’s Disease
check_url/4228?article_type=v

Controlling Parkinson’s Disease With Adaptive Deep Brain Stimulation
check_url/4228?article_type=v

Developing Neuroimaging Phenotypes of the Default Mode Network in PTSD: Integrating the Resting State, Working Memory, and Structural Connectivity
check_url/4228?article_type=v

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Read Article