A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:23Protein Expression and Purification
- 03:41DNA Purification
- 07:35Protein-DNA Complex Formation and Analysis
- 08:35Results: Crystal Structure of SeqA delta(41-59)-A25R Bound to Hemimethylated DNA
- 11:08Conclusion
Kristallstruktur av protein-DNA-komplex kan ge insikt i proteinfunktion, mekanism, liksom, arten av den specifika interaktionen. Här rapporterar vi hur du optimerar längden, sekvens och ändarna av duplex-DNA för samarbete kristallisation med<em> Escherichia coli</em> SeqA, en negativ regulator av replikering initiering.
Tags
DNA DuplexesCrystallizationSeqA-DNA ComplexesEscherichia Coli SeqANegative RegulatorDNA ReplicationPremature Reinitiation EventsHemimethylated GATC ClustersOrigin Of ReplicationReplication ForksHigher Ordered StructuresHigh Affinity ComplexesMinimal Functional UnitDimerGATC SequencesMolecular LevelStructural AnalysisProtein-DNA CrystallizationPacking InteractionsProtein-protein Interactions
