Journal
/
Immunology and Infection
/
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

DOI:
10.3791/4316-v

•

07:27 min

•

May 16, 2013

•

, , ,
1Malawi-Liverpool-Wellcome Trust Clinical Research Programme, 2Liverpool School of Tropical Medicine, 3Department of Microbiology, Division of Medical Parasitology,New York University School of Medicine

Chapters

  • 00:05Title
  • 01:20Preparaing of Platelet-rich Plasma (PRP) and Platelet-poor Plasma (PPP)
  • 02:17Parasite Culture
  • 02:58Thin Blood Film Slide Preparation
  • 03:57Plasmagel Flotation
  • 05:01Clumping Assay
  • 06:08Results: Platelet-mediated Clump Assay
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

This method investigates the platelet-mediated clumping phenotype of Plasmodium falciparum-infected erythrocytes (pRBC) in clinical isolates. This is performed by isolating and co-incubating platelet-rich plasma and a suspension of pRBC.

Tags

Platelet-mediated ClumpingPlasmodium Falciparum-infected ErythrocytesResource Poor SettingCerebral MalariaMicrovessels ObstructionP. Falciparum-parasitized Red Blood CellsSequestrationEndothelial CellsPost-capillary VenulesSplenic ClearanceLow Oxygen PressureCytoadherenceHost ReceptorsSevere Pathological Outcomes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4316?article_type=v

Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
check_url/4316?article_type=v

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
check_url/4316?article_type=v

A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti
check_url/4316?article_type=v

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
check_url/4316?article_type=v

Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
check_url/4316?article_type=v

A Protocol for Analyzing Hepatitis C Virus Replication
check_url/4316?article_type=v

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
check_url/4316?article_type=v

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
check_url/4316?article_type=v

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
check_url/4316?article_type=v

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Read Article