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Generation of Human induceret pluripotente stamceller fra perifert blod Brug af STEMCCA lentivirusvektor
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JoVE Journal Biology
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Generation of Human induceret pluripotente stamceller fra perifert blod Brug af STEMCCA lentivirusvektor

DOI:
10.3791/4327-v

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12:03 min

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October 31, 2012

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, , , , , , , , , , ,
1Center for Regenerative Medicine (CReM),Boston University School of Medicine, 2Department of Hematology,Children’s Hospital of Philadelphia, 3Center for Cellular and Molecular Therapeutics,Children’s Hospital of Philadelphia

Chapters

  • 00:05Title
  • 01:50Isolation and Expansion of PBMCs
  • 04:34Transduction of PBMCs with STEMCCA Lentivirus
  • 06:11Plating Transduced Cells onto MEFs
  • 08:41Picking and Expansion of iPSC Clones
  • 09:26Results: Human Induced Pluripotent Stem Cells
  • 11:26Conclusion

Summary

Automatic Translation

Automatic Translation

Her viser vi en enkel og effektiv protokol til generering af humane iPSCs 3-4 ml perifert blod under anvendelse af en enkelt lentiviral omprogrammering vektor. Omprogrammering af let tilgængelige blodlegemer lover at fremskynde udnyttelsen af ​​IPSC teknologi ved at gøre det tilgængeligt for et bredere forskningsmiljø.

Tags

Human Induced Pluripotent Stem CellsPeripheral BloodSTEMCCA Lentiviral VectorTranscription FactorsOct4Klf4Sox2CMycPluripotent StateEmbryonic Stem CellsImmune RejectionDisease Modeling StudiesPharmacological Compounds ScreeningRegenerative TherapiesTransgene-free IPSCsLung DiseasesSkin FibroblastsReprogrammingPeripheral Blood Cells

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