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1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center,Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Chapters
- 00:00Title
- 01:18Introduction
- 03:16Preparation of Enzyme Solution
- 04:06Human Dental Pulp Isolation
- 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
- 06:28Outgrowth of Stem Cells from Tissue Explants
- 07:27Immunophenotyping
- 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
- 12:21Dental Pulp Stem Cells Culture and Differentiation Results
- 14:14Conclusion
Das beschriebene Verfahren Isolierung und Charakterisierung von menschlichen Zahnpulpa Stammzellen (hDPSCs) unter Verwendung entweder<strong> Enzymatische Dissoziation von Zellstoff (DPSC-ED)</strong> Oder<strong> Direkte Folge von Stammzellen aus Pulpagewebe Explantate (DPSC-OG). Dann gefolgt von</strong><em> In vitro</em> Vergleichende Unterscheidung der beiden Arten von hDPSCs zu Odontoblasten.
Tags
IsolationCharacterizationComparative DifferentiationHuman Dental Pulp Stem CellsPermanent TeethTwo Different MethodsWisdom TeethRoutine Orthodontic TreatmentsHDPSCsProliferation PotentialMulti-lineage Differentiation CapacityAutologous TransplantationRegenerative MedicineMesenchymal Stem CellsMSC FeaturesImmunomodulatory EffectAllograft TransplantationIsolation ConditionsSurface MarkersDifferentiation CapacityImpacted Third Molar TeethDental Diamond Disk
