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1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center,Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Chapters
- 00:00Title
- 01:18Introduction
- 03:16Preparation of Enzyme Solution
- 04:06Human Dental Pulp Isolation
- 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
- 06:28Outgrowth of Stem Cells from Tissue Explants
- 07:27Immunophenotyping
- 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
- 12:21Dental Pulp Stem Cells Culture and Differentiation Results
- 14:14Conclusion
विधि या तो का उपयोग करके और मानव दंत लुगदी स्टेम सेल (hDPSCs) के अलगाव के लक्षण वर्णन वर्णित<strong> लुगदी के enzymatic पृथक्करण (डीपीएससी प्रवर्तन निदेशालय)</strong> या<strong> गूदा ऊतक explants से स्टेम कोशिकाओं का सीधा परिणाम (डीपीएससी ओग). तो द्वारा पीछा</strong><em> इन विट्रो में</em> ओडॉन्टोब्लास्ट में hDPSCs के दोनों प्रकार के तुलनात्मक भेदभाव.
Tags
IsolationCharacterizationComparative DifferentiationHuman Dental Pulp Stem CellsPermanent TeethTwo Different MethodsWisdom TeethRoutine Orthodontic TreatmentsHDPSCsProliferation PotentialMulti-lineage Differentiation CapacityAutologous TransplantationRegenerative MedicineMesenchymal Stem CellsMSC FeaturesImmunomodulatory EffectAllograft TransplantationIsolation ConditionsSurface MarkersDifferentiation CapacityImpacted Third Molar TeethDental Diamond Disk
