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1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center,Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Chapters
- 00:00Title
- 01:18Introduction
- 03:16Preparation of Enzyme Solution
- 04:06Human Dental Pulp Isolation
- 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
- 06:28Outgrowth of Stem Cells from Tissue Explants
- 07:27Immunophenotyping
- 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
- 12:21Dental Pulp Stem Cells Culture and Differentiation Results
- 14:14Conclusion
Den beskrivna metoden isolering och karakterisering av humana celler Dental Pulp Stem (hDPSCs) med hjälp av antingen<strong> Enzymatisk dissociation av massa (DPSC-DE)</strong> Eller<strong> Direkt utväxt av stamceller från explantat massa vävnad (DPSC-OG). Sedan följt av</strong><em> In vitro</em> Jämförande differentiering av båda typerna av hDPSCs till odontoblaster.
Tags
IsolationCharacterizationComparative DifferentiationHuman Dental Pulp Stem CellsPermanent TeethTwo Different MethodsWisdom TeethRoutine Orthodontic TreatmentsHDPSCsProliferation PotentialMulti-lineage Differentiation CapacityAutologous TransplantationRegenerative MedicineMesenchymal Stem CellsMSC FeaturesImmunomodulatory EffectAllograft TransplantationIsolation ConditionsSurface MarkersDifferentiation CapacityImpacted Third Molar TeethDental Diamond Disk
