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1Department of Biochemistry, Microbiology and Immunology,Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3CIHR Program in Neurodegenerative Lipidomics,University of Ottawa , 4Carleton Immersive Media Studio,Azrieli School of Architecture and Urbanism
Chapters
- 00:00Title
- 02:48Introduction
- 03:26Preparing Reagents
- 03:46Obtaining the Vaginal Smear
- 05:06Cytological Staining using Crystal Violet
- 05:46Vaginal Cytology
- 07:13Conclusion
Här beskriver vi hur du identifierar skede av murina reproduktiva (proestrus brunst, metestrus eller diestrus) genom enkel, icke-invasiv insamling och cytologisk bedömning av vaginala smear prover. Vi beskriver vidare hur vaginal cytologi speglar cirkulerande hormonella nivåer som ligger övergång genom murina reproduktiva cykeln.
Tags
Vaginal LavageCrystal Violet StainingVaginal Cytological EvaluationMouse Estrous Cycle StagingReproductive StatusMouse Models Of DiseaseHormonal RegulationPathological ChallengeProestrusEstrusMetestrusDiestrusOvarian Steroids17-beta-estradiolProgesteroneLuteinizing HormoneFollicle Stimulating HormoneProlactinCell TypologyNucleated Epithelial CellsCornified Squamous Epithelial CellsLeukocytesVaginal SmearsInvasivenessNon-invasive Protocol
