/
/
Peptide: MHC tetrameer-gebaseerde verrijking van epitoop-specifieke T-cellen
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:50Cell Isolation from Lymphoid Tissue
- 03:35Tetramer Staining
- 04:18Magnetic Enrichment
- 06:39Flow Cytometry
- 09:07Results: Calculation of MHC Tetramer-based Enrichment of Epitope-specific T Cells
- 13:15Conclusion
Dit protocol beschrijft het gebruik van peptide: MHC tetrameren en magnetische microbolletjes voor laagfrequente populaties van epitoop-specifieke T-cellen isoleren en analyseren door flowcytometrie. Deze methode maakt de directe bestudering van endogene T-celpopulaties plaats van<em> In vivo</em> Experimentele systemen.
Tags
Peptide:MHC Tetramer-based EnrichmentEpitope-specific T CellsT Cell Antigen Receptor SpecificityFunctional ResponseProliferationCytokine ProductionActivation MarkersTCR Transgenic Adoptive Transfer ModelMonoclonal T CellsHistocompatible HostsCongenic Marker AntibodyExperimental ArtifactsFunctional HeterogeneityPolyclonal Population
