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新鲜组织分离正常和癌症相关的成纤维细胞的荧光激活细胞分选（FACS）

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JoVE Journal Biology

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)

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新鲜组织分离正常和癌症相关的成纤维细胞的荧光激活细胞分选（FACS）

DOI:

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11:31 min

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January 14, 2013

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Yoray Sharon, Lina Alon, Sarah Glanz, Charlotte Servais, Neta Erez

¹Department of Pathology, Sackler School of Medicine,Tel Aviv University

Chapters

00:05Title
01:45Dissecting Mammary and Skin Tissue from Mice
03:34Preparation of Mammary Gland/Skin Single Cell Suspension
04:49Staining Cells
07:07Isolation of Cells by FACS
08:47Results: Isolation of Highly Enriched Populations of Tissue Fibroblasts by FACS
10:49Conclusion

Summary

Automatic Translation

癌相关成纤维细胞（CAFS）信号，促进细胞增殖，血管生成和炎症促进肿瘤的发生，生长和发展。在这里，我们描述了一种方法，分离出纯净的新鲜小鼠和人体组织的正常成纤维细胞和纤维母细胞分选，表面标志使用PDGFRα的人口。

Tags

Isolation Normal Fibroblasts Cancer-associated Fibroblasts Fluorescence Activated Cell Sorting FACS Tumor Stroma Breast Carcinoma Poor Prognosis Myofibroblast Marker α-SMA Bone Marrow-derived Cells Tumor Microenvironment Tumor Initiation Tumor Growth Tumor-promoting Inflammation CAF-specific Markers Heterogeneity Gene Expression Profiling

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