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JoVE Journal Bioengineering
Quantitative FRET (transfert de Förster Resonance Energy) Analyse de la protéase SENP1 Kinetics Détermination
 
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Quantitative FRET (transfert de Förster Resonance Energy) Analyse de la protéase SENP1 Kinetics Détermination

Article DOI: 10.3791/4430-v • 17:12 min • February 21st, 2013
February 21st, 2013 •
1, 1
1Department of Bioengineering, Bourns College of Engineering, University of California, Riverside

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Une nouvelle méthode qui analyse quantitative de FRET (Förster Resonance Energy Transfer) des signaux est décrite pour l'étude de la cinétique enzymatique.

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Bioingénierie Numéro 72 biochimie biologie moléculaire protéines quantitative analyse FRET QFRET cinétique enzymatique analyse SENP SUMO un plasmide d'expression de protéine, purification de protéines dosage de la protéase l'analyse quantitative
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