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En molekylær Avlesning av Long-term olfactory Tilpasning i<em> C. elegans</em
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Chapters
- 00:05Title
- 01:57Cultivation and Maintenance of Animals for Nuclear Translocation Assays
- 03:26Long-term Odor Induced Nuclear Translocation Assays
- 05:44Scoring the Odor-induced Nuclear Translocation Event
- 08:54Results: Molecular Readout of Long-term Olfactory Adaptation in C. elegans
- 11:09Conclusion
Her beskriver vi en molekylær avlesning av langsiktig olfactory tilpasning i<em> Caenorhabditis elegans</em>. Proteinkinase G, EGL-4, som er nødvendig for stabile tilpasningstiltak responser i primær sensorisk neuron pair kalt AWC. Under langvarig lukt eksponering EGL-4 translocates fra cytosol til kjernen av AWC.
Tags
Molecular ReadoutLong-term Olfactory AdaptationC. ElegansSensory NeuronsAdaptation ResponseOlfactory CircuitGenetic ScreenMutantsVolatile OdorsAmphid Wing Cells Type C (AWC) Sensory NeuronsChemotaxis Behavioral AssayShort-term Olfactory AdaptationLong-term Olfactory AdaptationProtein Kinase G (PKG)EGL-4 Protein
