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Imaging Analyse av Neuron til gliaceller samhandling i Microfluidic Kultur Platform (MCP)-baserte Neuronal Axon og gliaceller Co-kultur System
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Chapters
- 00:05Title
- 01:27Assembly of the Microfluidic Culture Chamber (MCP)
- 02:43Preparation of Neuronal Culture
- 03:33Induction of Neuronal Axons in Assembled MCP
- 04:48A Compartmentalized Co-culture System: Adding Cultured Astrocytes
- 06:15Results: Axon-induced GLT1 Promoter Activation in Astrocytes
- 09:07Conclusion
Denne studien beskriver prosedyrene for å sette opp en ny neuronal axon og (astro) gliaceller co-kultur plattform. I dette samarbeidet kultur systemet, blir manipulering av direkte interaksjon mellom en enkelt axon (og eneste glial celle) gjennomførbart, slik mekanistisk analyse av gjensidig nervecelle til glial signalering.
Tags
Imaging AnalysisNeuronGlia InteractionMicrofluidic Culture PlatformMCP-based Neuronal Axon And Glia Co-culture SystemSignaling PathwaysCentral Nervous System (CNS)Neuron And Glia Mixed CulturesNeuronal CompartmentsAxonDendriteSomaCulture SystemSoluble FactorsDirect Membrane Contact SignalsSingle AxonGlial Cell
